Distinct Kinesin-14 mitotic mechanisms in spindle bipolarity
نویسندگان
چکیده
منابع مشابه
Aurora A, mitotic entry, and spindle bipolarity.
The kinase Aurora-A (Aur-A), which is enriched at centrosomes, is required for centrosome maturation and accurate chromosome segregation, and recent work implicates centrosomes as sites where the earliest activation of cyclin B1-cdc2 occurs. Here, we have used Xenopus egg extracts to investigate Aur-A's contribution to cell cycle progression and spindle morphology in the presence or absence of ...
متن کاملPolycystic kidney disease protein fibrocystin localizes to the mitotic spindle and regulates spindle bipolarity.
Autosomal recessive polycystic kidney disease (ARPKD) is a significant hereditary renal disease occurring in infancy and childhood, which presents with greatly enlarged echogenic kidneys, ultimately leading to renal insufficiency and end-stage renal disease. ARPKD is caused by mutations in a single gene PKHD1, which encodes fibrocystin/polyductin (FPC), a large single transmembrane protein gene...
متن کاملProbing Spindle Assembly Mechanisms with Monastrol, a Small Molecule Inhibitor of the Mitotic Kinesin, Eg5
Monastrol, a cell-permeable small molecule inhibitor of the mitotic kinesin, Eg5, arrests cells in mitosis with monoastral spindles. Here, we use monastrol to probe mitotic mechanisms. We find that monastrol does not inhibit progression through S and G2 phases of the cell cycle or centrosome duplication. The mitotic arrest due to monastrol is also rapidly reversible. Chromosomes in monastrol-tr...
متن کامل[Kinesin-14 leaps to pole position in bipolar spindle assembly].
Formation of a mitotic spindle occurs almost effortlessly as cells cycle and is essential for chromosome segregation. Early in a cell cycle, the replication of centrosomes or spindle pole bodies at G1/S leaves mother and daughter poles in close association until forced apart into a bipolar arrangement at mitotic onset. Kinesin-like proteins (Klps) generate a variety of forces that contribute to...
متن کاملKinesin-Related Proteins at Mitotic Spindle Poles: Function and Regulation
other highly conserved mitotic motor family is the minus-University of California end–directed Kar3 family, which appears to play an an-San Francisco, California 94143-0450 tagonistic role to the bimC family of KRPs (reviewed by Sawin and Endow, 1993). Owing to space limitations, The mitotic spindle, which consists of a dynamic array we will not discuss this family of KRPs or any of the of micr...
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ژورنال
عنوان ژورنال: Cell Cycle
سال: 2009
ISSN: 1538-4101,1551-4005
DOI: 10.4161/cc.8.21.9970